Ferritin-labelled Antibody Study of RD-114 Virus
نویسندگان
چکیده
منابع مشابه
Purification and immunological characterization of the major internal protein of the RD-114 virus.
The major internal protein of the RD-114 virus that appeared in a human tumor cell line, RD, after passage through fetal cats was purified by isoelectric focusing and used to prepare antibody in guinea pigs. The protein has a molecular weight of 33,500 in sodium dodecyl sulfate-polyacrylamide gels, and an isoelectric point of 9.1. This contrasts with a molecular weight of 25,000 and an isoelect...
متن کاملPresence of infectious RD-114 virus in a proportion of canine parvovirus isolates.
We recently found that certain canine live attenuated vaccines produced using `non-feline' cell lines were contaminated with an infectious feline endogenous retrovirus, termed RD-114 virus. We suspected that RD-114 virus may have contaminated the seed stock of canine parvovirus (CPV) during the production of the contaminated vaccines. In this study, we collected stock viruses of CPVs propagated...
متن کاملEndogenous RD-114 virus genome expression in malignant tissues of domestic cats.
Endogenous xenotropic cat type C virus (RD-114)- and infectious feline leukemia virus (FeLV)-specific gene expressions were measured in spontaneous sarcomas carcinomas, and nonmalignant cat tissues by molecular hybridization for virus-specific RNA and competition radio-immunoassays for the major internal protein (p30) of these two viruses. The results indicate that RD-114 gene expression in sar...
متن کاملSuppressive effects of interferon on syncytium formation by RD-114 virus in human transformed cells.
The RD-114 virus rapidly induced syncytia in the human transformed cell lines, RSa, RSb and IFr. Treatment of the virus with heat or ultrasonic vibration completely eliminated the syncytium-forming activity. Irradiation with u.v.-light or treatment with beta-propiolactone (BPL) reduced but did not completely destroy the activity. Pre-treatment of the cells for 16 h with 25 to 500 units/ml of hu...
متن کاملFeline leukemia and RD-114 virus group-specific proteins: comparison of amino terminal sequence.
The major internal virion polypeptide from feline and RD-114 type C viruses has been subjected to amino terminal sequence analyses with the Beckman automated sequencer. These proteins, as well as their homologs in rat and mouse viruses, begin with the sequence prolylleucylarginyl (Pro-Leu-Arg). Virus RD-114 differs from conventional feline type C viruses that show about 80 percent relatedness b...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1974
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-22-2-277